| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.066550 |
| Chromosome: | chromosome 12 |
| Location: | 6313646 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g536800 | CGL52 | (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); Conserved in the Green Lineage | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGGTAAAGGAGTATGGAGAAGTCGGAGCAAGTTCGAGGATTCTGTTT |
| Internal bar code: | GAGGCTCTTACAGACACGTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 427 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCATTCTGCTGCTCCTCCG |
| Suggested primer 2: | GTTGCTTTCTCCCTAGGCGT |