| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.066551 |
| Chromosome: | chromosome 2 |
| Location: | 3955163 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g102450 | (1 of 1) PTHR22847//PTHR22847:SF358 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGATAAATCCTACCCAGTTGGCTCTTGCTTAAGGAAGAGACAGGAAAC |
| Internal bar code: | TATAGCCGTGGTTCTATAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1353 |
| LEAP-Seq percent confirming: | 54.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTGCGTCACAACAGGTA |
| Suggested primer 2: | TCCGGGACAGCAGACTTTTC |