| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.066756 |
| Chromosome: | chromosome 16 |
| Location: | 1499165 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652950 | (1 of 1) K11274 - chromosome transmission fidelity protein 4 (WDHD1, CTF4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTATGACCTTGCGTCGAGAGCCCCCGCCTCTAGTGAAGGGGAAGAAGAA |
| Internal bar code: | TTGATATGATGTATTACTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3333 |
| LEAP-Seq percent confirming: | 95.5224 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGCTTCCAACGTTGAAAG |
| Suggested primer 2: | ACTGCCAAAGGACGCATGTA |