Insertion junction: CLIP2.066815_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.066815
Chromosome:chromosome 6
Location:1413325
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g259750 3'UTR
Cre06.g259800 CWC22 (1 of 1) K13100 - pre-mRNA-splicing factor CWC22 (CWC22); Cwc22 pre-mRNA splicing factor 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGAGAACAAGACGTGTAGCTACACTGTTATAGTCTCACCCACATTCCA
Internal bar code:CCACGTAGGTTCTCTCCCTAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1436
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTTGCTTGGTTCGTGTC
Suggested primer 2:GTGATTGTATTGGCGCGTCC