Insertion junction: CLIP2.066930_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.066930
Chromosome:chromosome 6
Location:6184299
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g291950 MRS4,MRS3 Mg2+ transporter protein; (1 of 3) K16075 - magnesium transporter (MRS2, MFM1) intron
Cre06.g292000 (1 of 1) PF00790//PF03127 - VHS domain (VHS) // GAT domain (GAT) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCGGGAGCCAAGCTAAGCGCAAGCCAAGCGCAGAAGCGCGCACGCATG
Internal bar code:GGTGGTAGTTAGAGACTATGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1777
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGAGGCGTGTTTGGTTG
Suggested primer 2:CACACACACACACACACACG