| Insertion cassette: | CIB2 | 
| Side of cassette: | 3' | 
| Strand: | - | 
| Strain: | CLIP2.067680 | 
| Chromosome: | chromosome 13 | 
| Location: | 4220250 | 
| Confidence (%): | 63 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre13.g591700 | TTL15 | (1 of 7) 1.2.1.24 - Succinate-semialdehyde dehydrogenase (NAD(+)); Putative tubulin tyrosine ligase | CDS/intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATCATCGCGCTTGGCGCCCGTGGCTGCGTCCAGCTTGGGCGGCCCGCC | 
| Internal bar code: | CTACGATGTGAAGATGCTTCAG | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 116 | 
| LEAP-Seq percent confirming: | 100.0 | 
| LEAP-Seq n confirming: | 1 | 
| LEAP-Seq n nonconfirming: | 0 | 
| LEAP-Seq n unique pos: | 1 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGGAGAGCCTGATGACGG | 
| Suggested primer 2: | CACCTGCGAACAACACAAGG |