| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068062 |
| Chromosome: | chromosome 2 |
| Location: | 827238 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g078750 | PUS3 | RNA pseudouridine synthase; (1 of 1) K15452 - tRNA pseudouridine synthase 2 [EC:5.4.99.-] (PUS2) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCGACGATGAGTTTCTTCCCAGACCTTAAGTCTGTAGAGATAACGAA |
| Internal bar code: | CTGGGTTTTTGGGTTTTCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2320 |
| LEAP-Seq percent confirming: | 97.8261 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATGCTTTGAAAGGCGCGT |
| Suggested primer 2: | AGGTCAAGGCCTGTTTGGAG |