| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.068470 |
| Chromosome: | chromosome 11 |
| Location: | 4222383 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481750 | GAS30 | (1 of 24) IPR003882//IPR024616 - Pistil-specific extensin-like protein // Pherophorin; Hydroxyproline-rich glycoprotein, stress-induced | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGATGTTCTCTAAAGAAACAGCACCAAGGTGTGTGGTGCTGCTCAGGCG |
| Internal bar code: | TTTTACATTTCGGTCTCCAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3076 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 81 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAGCCTTCCACCCGAGAGT |
| Suggested primer 2: | CATCCCTGCATGCATGTTCG |