Insertion junction: CLIP2.068754_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.068754
Chromosome:chromosome 10
Location:2743573
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g438050 KAS3,mtKAS (1 of 1) PTHR11712//PTHR11712:SF273 - POLYKETIDE SYNTHASE-RELATED // 3-OXOACYL-[ACYL-CARRIER-PROTEIN] SYNTHASE, MITOCHONDRIAL; Putative mitochondrial beta-ketoacyl-ACP synthase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGCGCAGCCTACTTTTCCCATGCGATCTTGTCTTACGCGCCCCGCTG
Internal bar code:TGTAAACAGACACCCCTGCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1325
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCACACTGACCCGTATGG
Suggested primer 2:AGCAGCACATCTGAATGCCT