| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.068843 |
| Chromosome: | chromosome 10 |
| Location: | 1576840 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429017 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAATGCCTTATCAGCTGGGCCCGGATTGGCCCCGGCGGCCTCGTCGCA |
| Internal bar code: | ATAGAGCTGCAAAGTATATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4085 |
| LEAP-Seq percent confirming: | 64.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGACATTTCGGCAGTTGC |
| Suggested primer 2: | AGTAAAGGGTGTGCGATGGG |