| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.068888 |
| Chromosome: | chromosome 7 |
| Location: | 3468348 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335700 | 5'UTR | ||
| Cre07.g335750 | IFT88 | (1 of 1) K16474 - intraflagellar transport protein 88 (IFT88); Intraflagellar Transport Protein 88 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCTGTAAAGGTCGAGACCAACATTATTTCAACTGGATTCGATCAACTT |
| Internal bar code: | GAGAATAGTCAACCTGTGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3653 |
| LEAP-Seq percent confirming: | 94.6429 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACCTACAGGCAGGCAGC |
| Suggested primer 2: | GCGTAAAGCCATTACCGCTG |