Insertion junction: CLIP2.069133_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.069133
Chromosome:chromosome 14
Location:2381803
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre14.g623200 LAP1 Leucyl aminopeptidase; (1 of 1) 3.4.11.1//3.4.11.23 - Leucyl aminopeptidase / Peptidase S // PepB aminopeptidase / Peptidase B 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCTCACCACCGTTTTCCATCCCGCCAGGCCCTCACGCGGTCACGCCG
Internal bar code:CCTTAAAACGGGATTGCTGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:390
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGTTCCTGCAGGAGTTCG
Suggested primer 2:GAGACGAACACAACCGGAGT