| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.069291 |
| Chromosome: | chromosome 3 |
| Location: | 7442954 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g205900 | DCA1 | (1 of 1) 4.1.1.50 - Adenosylmethionine decarboxylase / S-adenosyl-L-methionine decarboxylase; S-adenosylmethionine decarboxylase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCGAATTGATTGTGCTTGTGTGGAGCGAGGCACGCGACTGTGTGGTT |
| Internal bar code: | ACCGTGCGATTGGATGGGTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2436 |
| LEAP-Seq percent confirming: | 98.7654 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 81 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTTGCCAGGCGGAAAAA |
| Suggested primer 2: | GCAGAATCACTTGAGCCCCT |