| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069517 |
| Chromosome: | chromosome 10 |
| Location: | 593474 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g421600 | TST2 | (1 of 1) PTHR11451//PTHR11451:SF32 - TRNA SYNTHETASE-RELATED // THREONYL-TRNA SYNTHETASE / THREONINE--TRNA LIGASE-RELATED; Threonyl-tRNA synthetase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGCCACTCATTCAGCATGGAGTCAGCAATTCAATTCCTGGGTTCGCGC |
| Internal bar code: | CTACAAATAGAAATCGTTCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1201 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 45 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAAGTGCGGAATCGAGC |
| Suggested primer 2: | CCTATCTTCCCATGCCGTCC |