Insertion junction: CLIP2.069721_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.069721
Chromosome:plastome
Location:172305
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802328 psbJ,2716960,ChreCp063 (1 of 1) K02711 - photosystem II PsbJ protein (psbJ); photosystem II reaction center protein J CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGTCCAGTAGTTCCAGTATTAGACATAAATATAAAAATGGTTTCATTA
Internal bar code:GGGGTTCCCCATACGACACTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:55
LEAP-Seq percent confirming:11.1111
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTTCGGGCAACTAAAGT
Suggested primer 2:CAGCCTCACAGTTTGCCAAG