| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.069848 |
| Chromosome: | chromosome 6 |
| Location: | 1621077 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261700 | (1 of 1) K14815 - mRNA turnover protein 4 (MRT4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCACGGGTGTGGCGGCGGACCGAGTAGTCGTGGTGCAGTAGCTAGAGC |
| Internal bar code: | GATTTGATCAGTCAGGCACCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1906 |
| LEAP-Seq percent confirming: | 31.25 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATATGCTTCGCGCTGACGT |
| Suggested primer 2: | TTCCCACCTCTCCTACTCCG |