| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.070122 |
| Chromosome: | chromosome 5 |
| Location: | 592021 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g244700 | PFH15,P4H-1,P4G15,PHX10 | Prolyl 4-hydroxylase 15; (1 of 5) PTHR10869//PTHR10869:SF55 - PROLYL 4-HYDROXYLASE ALPHA SUBUNIT // OXOGLUTARATE/IRON-DEPENDENT OXYGENASE | 3'UTR |
| Cre05.g244750 | (1 of 1) K13205 - A1 cistron-splicing factor AAR2 (AAR2, C20orf4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAAGTGGCTGAGCAGGCAGCTGCAGCTTCGGACCTTCGGTGAATCGG |
| Internal bar code: | CCAGTTGTTTAATTCAACGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 91.3043 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGCCGTGATTTACTGTCA |
| Suggested primer 2: | CCGCATATGCCATCCACTCT |