| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070126 |
| Chromosome: | chromosome 8 |
| Location: | 464988 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358950 | (1 of 1) PF09731 - Mitochondrial inner membrane protein (Mitofilin) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCGGCAGTAGTGATGTGTGTGGGTCGATGTAAACCCTCCAGGGTCCTA |
| Internal bar code: | CCCTCAGGCCATCGAATCCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5441 |
| LEAP-Seq percent confirming: | 81.3953 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGTTTGGAGGGTTTGCC |
| Suggested primer 2: | ATTGTTGTGGTGGTGGTGGT |