| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.070225 |
| Chromosome: | chromosome 4 |
| Location: | 680097 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217951 | LCI33 | Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTATCAAGGTCTTTGCTGAGCCCTACACGCGTCTTATCACACAACATG |
| Internal bar code: | GGGCCGTTTTAGATCTTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3706 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCAGGTGCTTTTTCGTTC |
| Suggested primer 2: | GGAGTCGGCGTATTACTGGG |