Insertion junction: CLIP2.070258_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.070258
Chromosome:plastome
Location:63840
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802291 ChreCp027,psbZ,2716976 photosystem II reaction center Z protein; (1 of 1) K02724 - photosystem II PsbZ protein (psbZ) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGAGAATACTGCACCTTTATTGTCAGTCCAACCATTAGGAGTTGCAAA
Internal bar code:TGGAGCACGGGACGCGCACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:167
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGAACAAACACGGTGCAACG
Suggested primer 2:CGATAGGCGAGGCATCACAT