Insertion junction: CLIP2.070272_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.070272
Chromosome:chromosome 12
Location:9321561
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g542050 3'UTR
Cre12.g542100 (1 of 2) 3.4.21.26 - Prolyl oligopeptidase / Prolyl endopeptidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCATGCATTGCCCTCAACCAGCACAGGTTTCAGTTGCCAGATCGTCAG
Internal bar code:TGCTTAGGACTGGACAACATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1817
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGTGCATGAACGCCAAA
Suggested primer 2:GCGGCACTTTACAACAACGT