| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.070746 |
| Chromosome: | chromosome 12 |
| Location: | 7175807 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559350 | SDR23 | Short-chain dehydrogenase/reductase; (1 of 1) 1.1.1.101 - Acylglycerone-phosphate reductase / Palmitoyldihydroxyacetone-phosphate reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCTCGTGAGTGTCGTGACCTGCTCCATCAAACTCAAGAAACGCTCAA |
| Internal bar code: | GAAGCTTTTAATAGGGAAATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 737 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCAGGTGAGAGCAGGAT |
| Suggested primer 2: | GCGCTCTTCACAATGGCAAA |