| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.071025 |
| Chromosome: | chromosome 2 |
| Location: | 7015020 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389689 | ASD1,ASSD1 | Aspartate semialdehyde dehydrogenase; (1 of 1) 1.2.1.11 - Aspartate-semialdehyde dehydrogenase / L-aspartate-beta-semialdehyde dehydrogenase | 5'UTR |
| Cre09.g389726 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGGAAGAAGGAAGAAGAGGAGAAGCGCCGTAAGCAGGACGAAGAGCT |
| Internal bar code: | CGTTTTCGTGCTCGAGAACAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4248 |
| LEAP-Seq percent confirming: | 68.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCGCATGGCTTGAAAGAC |
| Suggested primer 2: | CGAGAAACCTGAGTGCGAGT |