| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.071471 |
| Chromosome: | chromosome 11 |
| Location: | 2527202 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095101 | (1 of 1) PTHR24012:SF459 - RNA-BINDING PROTEIN 4F | 3'UTR | |
| Cre02.g095102 | VPS5C | (1 of 4) PTHR10555:SF84 - SORTING NEXIN-8; Subunit of Retromer complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTGAGATGTGTTTGGCACTGCATGAACGCTGCTGTGTTGTTTGCGTG |
| Internal bar code: | AATTTTGATAACGGTTACACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 820 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAAAGTGAGCCAGTCGA |
| Suggested primer 2: | CAGTGGTGGTAGTGGCAGAG |