| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072005 |
| Chromosome: | chromosome 3 |
| Location: | 5139091 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g180800 | HDA16 | Histone deacetylase complex protein; (1 of 1) K11644 - paired amphipathic helix protein Sin3a (SIN3A) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTCAGTTCCGCGCTCACCGAGCACTCACATACTAACAAGCAAAAGCCT |
| Internal bar code: | ACATTATCTCCATGACCGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 18.1818 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTCACTGAGAACGAGC |
| Suggested primer 2: | GTTTTCCGAACACGCAGCTT |