Insertion junction: CLIP2.072161_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072161
Chromosome:chromosome 9
Location:5339604
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g407700 CEP1 (1 of 1) K16292 - KDEL-tailed cysteine endopeptidase [EC:3.4.22.-] (CEP, CYSEP); Cysteine endopeptidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGTGGATCTCGCACTACATTTCTGGTTTGGACGGCGATTCCTGAACTA
Internal bar code:AACGAAGAGGCCTCCAATTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:215
LEAP-Seq percent confirming:28.0
LEAP-Seq n confirming:14
LEAP-Seq n nonconfirming:36
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGAACCAGAGACAGGGTG
Suggested primer 2:CATGCAGGTGGCTCTGAAGA