| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072244 |
| Chromosome: | chromosome 12 |
| Location: | 3980034 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGAACAGGAAGGTGGAGTGCATCCGTGCAATGGGATAGGGTAAGGTC |
| Internal bar code: | TCTTATATAATCAAGCAGTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1616 |
| LEAP-Seq percent confirming: | 89.7959 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTAGGTGTTGAGCAGGCTG |
| Suggested primer 2: | CATGGTTGCTTGTTTCCGCA |