| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072831 |
| Chromosome: | chromosome 7 |
| Location: | 4597719 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344250 | CSG9 | Chlamydomonas specific family G protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCGAGGTACATTATTGCCCATCTCCTTCCAAGGCCGATGCGTCAGCATG |
| Internal bar code: | GCCAGCTTAATCTTCGGTAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1760 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 55 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTGTTTGACGACGATGA |
| Suggested primer 2: | AGGATGGAGGTAGGGCATGT |