| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073096 |
| Chromosome: | chromosome 12 |
| Location: | 3042476 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501600 | BLZ8 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2); bZIP transcription factor | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGAGGACGAGGGAGGAGGATGCAAGGGACTTCTGTACGTTTAATGCCTT |
| Internal bar code: | TCATTATGGATATAAAAGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1547 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATTGCGAAACACGGCTAG |
| Suggested primer 2: | GGTATCCATGCTGCTGCTGT |