| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073280 |
| Chromosome: | chromosome 16 |
| Location: | 6035701 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678100 | VPS28 | (1 of 1) K12184 - ESCRT-I complex subunit VPS28 (VPS28); Subunit of the ESCRT-I complex | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGGTGTGGGAATGCATGAGACAGGGCGCTGAATGAGGGCGGATGGGC |
| Internal bar code: | GAACAGTTCTTAGATATAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 406 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGAGGTCGAGCAGGATGG |
| Suggested primer 2: | CACGTCAGAAAAAGCGGCAA |