| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.073653 |
| Chromosome: | chromosome 3 |
| Location: | 3607793 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168050 | GTF2 | Global Transcription factor; (1 of 3) IPR001487//IPR027353 - Bromodomain // NET domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCAGTACTCGGGTGGGTTGGGGGCGAGCCTTGGAGGCCTGCTGTTGT |
| Internal bar code: | TGTAACGGTGGACGTTGAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4416 |
| LEAP-Seq percent confirming: | 98.3871 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCAGGAATGGCTTGACC |
| Suggested primer 2: | ATACTTTTGTGAGGGGGCCG |