| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.073685 |
| Chromosome: | chromosome 1 |
| Location: | 4021647 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026450 | SRP35,SRS1 | Serine/arginine-rich pre-mRNA splicing factor; (1 of 2) K12890 - splicing factor, arginine/serine-rich 1/9 (SFRS1_9) | 5'UTR |
| Cre01.g026500 | ASP3 | (1 of 2) 3.4.23.12 - Nepenthesin / Nepenthes aspartic proteinase; Pepsin-type aspartyl protease | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATACAAACACCTGCTAAAACAATTTCCAGGCATTCCTCAATTAAACGC |
| Internal bar code: | GTATGACCATCACCGGGTGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4536 |
| LEAP-Seq percent confirming: | 64.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGTCCCTCTGGGTCCTCTC |
| Suggested primer 2: | CTCATGAAACGGCTCGCATG |