Insertion junction: CLIP2.074010_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.074010
Chromosome:chromosome 10
Location:3538816
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g445000 SLT2 Sodium/sulfate co-transporter 2; (1 of 2) PF00939//PF02080 - Sodium:sulfate symporter transmembrane region (Na_sulph_symp) // TrkA-C domain (TrkA_C) 3'UTR
Cre10.g445050 SLT3 (1 of 1) IPR001898//IPR004680//IPR006037 - Sodium/sulphate symporter // Citrate transporter-like domain // Regulator of K+ conductance, C-terminal; Sodium/sulfate co-transporter 3 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAAGAAAAAGCGGCACTCGGTCCGCGCAACTTCTTTGTCTCAAGTTGGGA
Internal bar code:AGGCCCCAGGAGGACGATCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3626
LEAP-Seq percent confirming:45.4545
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGCAGGCTTGGTGATGT
Suggested primer 2:ATCACCGGGCGCTTTTCTAA