Insertion junction: CLIP2.074029_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.074029
Chromosome:chromosome 11
Location:2526936
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095101 (1 of 1) PTHR24012:SF459 - RNA-BINDING PROTEIN 4F 3'UTR
Cre02.g095102 VPS5C (1 of 4) PTHR10555:SF84 - SORTING NEXIN-8; Subunit of Retromer complex 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCGTCTCCGCAAACATTGCCGCCAGCTGCTTCCTCCTCGCTTCTACTC
Internal bar code:TTCTTCCGTCACATCAGCTTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:415
LEAP-Seq percent confirming:93.1034
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:TGTCCATCGCTCCCTCTACA
Suggested primer 2:AGACGCAGAAGTGGACGAAG