| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.074236 |
| Chromosome: | chromosome 1 |
| Location: | 4084620 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027100 | IMP2 | Mitochondrial inner membrane signal peptidase; (1 of 1) K09648 - mitochondrial inner membrane protease subunit 2 (IMP2) | 3'UTR |
| Cre01.g027150 | CPLD46,HEL5 | Conserved in the plant lineage and diatoms; (1 of 2) PTHR24031//PTHR24031:SF249 - RNA HELICASE // ATP-DEPENDENT DNA HELICASE RECG | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTCCAAAGTGAACGGGACCTTTCCATGCGCCACGCCGAAATCACACC |
| Internal bar code: | GAATGGCACCTTGATAGCCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4058 |
| LEAP-Seq percent confirming: | 97.7778 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCGTTCCAACCTGCATG |
| Suggested primer 2: | GAGTGGAGGGGGTTTGATGG |