| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074252 |
| Chromosome: | chromosome 1 |
| Location: | 2668198 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g016050 | PRP8 | (1 of 1) K12856 - pre-mRNA-processing factor 8 (PRPF8, PRP8); Splicing factor, component of the U5 snRNP and of the spliceosome | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCCACCATGCAAACGACCCGACACGGCCTGCAACTGCCTGCAACTCGT |
| Internal bar code: | GGCACCCTTATGTCCAGGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4849 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAAGAGGAGAACAAGGGCC |
| Suggested primer 2: | GCGAGATAGAGACGAACGCA |