| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.074350 |
| Chromosome: | chromosome 9 |
| Location: | 4472487 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400812 | (1 of 2) PF12325 - TATA element modulatory factor 1 TATA binding (TMF_TATA_bd) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAAAGCACCGCGTGTGGGCGCACCCCAGCTAGTACCGGTACTAGCGC |
| Internal bar code: | AAGTAAGCTGTGGTCGTATTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1914 |
| LEAP-Seq percent confirming: | 82.1429 |
| LEAP-Seq n confirming: | 92 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGCAAACATCCACCAC |
| Suggested primer 2: | TAGTGCGGCTGTTGACAACT |