| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.074879 |
| Chromosome: | chromosome 16 |
| Location: | 1173837 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650450 | FAP219 | Flagellar Associated Protein 219 | 5'UTR |
| Cre16.g650500 | (1 of 1) K06195 - ApaG protein (apaG) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCAAAATGATGCGCAGGTCGGCTTCAACTCAAAAGACTAGCCGCTGT |
| Internal bar code: | AGAGTTCTGCAAACATCGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1556 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCCTCCACGTTTGGTAT |
| Suggested primer 2: | GGTTAAGAGGGGAAACGGGG |