Insertion junction: CLIP2.074886_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.074886
Chromosome:plastome
Location:67669
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802292 ccsA,ChreCp028,2717027,ycf5 (1 of 1) PTHR30071//PTHR30071:SF8 - HEME EXPORTER PROTEIN C // CYTOCHROME C BIOGENESIS PROTEIN CCSA; cytochrome c biogenesis protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGAGTAATGTTCCTCCCTTTATAGGAGATTGACATTCTTTGAAAAAGA
Internal bar code:AGGGATATCGATACATCTAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:128
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTTCTTGTGCGAACAAGG
Suggested primer 2:AAGAAACACCCAACAGCCCA