| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.074980 |
| Chromosome: | chromosome 10 |
| Location: | 5457675 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g457550 | ATG18 | (1 of 1) K17908 - autophagy-related protein 18 (WIPI, ATG18); Autophagy related | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTGGTATGCAGGGCACGGTGCGCATTTACGACCTGGCTCAGGAGGGT |
| Internal bar code: | CATTGATATCAGTTGGCAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 465 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCACGGAGTATCGCATAA |
| Suggested primer 2: | CGTAGCCCTGCATACACGAT |