Insertion junction: CLIP2.075120_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075120
Chromosome:chromosome 6
Location:3805109
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre06.g278174 (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCGCGTGATGATCCCTTGCCTTTTAGCTTCAGCAGCTGGACCGCCACC
Internal bar code:ATACTTATCGACCAAATGCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:324
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCACCAGCTGTGCTAGA
Suggested primer 2:CCACCAGGGTCGGTTTACTC