Insertion junction: CLIP2.075140_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.075140
Chromosome:plastome
Location:172440
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802328 psbJ,2716960,ChreCp063 (1 of 1) K02711 - photosystem II PsbJ protein (psbJ); photosystem II reaction center protein J 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACAGAATTAGCCCTTAACTGCTTGGCATTTTTGTATTGCAAGGTTGTA
Internal bar code:CATATAGACATGAACTCGTACT

Confirmation - LEAP-Seq

LEAP-Seq distance:105
LEAP-Seq percent confirming:4.65116
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:41
LEAP-Seq n unique pos:43

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCTCACAGTTTGCCAAG
Suggested primer 2:CCCCTTCGGGCAACTAAAGT