| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.075250 |
| Chromosome: | chromosome 6 |
| Location: | 6593886 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g294400 | NIS1 | (1 of 1) K06171 - nicastrin (NCSTN); Nicastrin | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAAGTCATGTCCGTGCACAACAGGGGTCAATCAGCAGCGTCACATGCC |
| Internal bar code: | TGCGTGAGCGATGAGGTACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1936 |
| LEAP-Seq percent confirming: | 97.5 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGTTACCAAGCAAGGTTG |
| Suggested primer 2: | CCCCAAACTTTCTGCATGGC |