| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075424 |
| Chromosome: | chromosome 6 |
| Location: | 2718184 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270850 | SRH12 | (1 of 1) K10841 - DNA excision repair protein ERCC-6 (ERCC6, CSB, RAD26); SNF2-related DNA/RNA helicase | 5'UTR |
| Cre06.g270900 | SUV3 | Mitochondrial DEAD-box DNA/RNA helicase; (1 of 1) K17675 - ATP-dependent RNA helicase SUPV3L1/SUV3 [EC:3.6.4.13] (SUPV3L1, SUV3) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGTAACTAATAATTAAGTTTGTATTGCCTACGGCTATGAGAGAAAGC |
| Internal bar code: | TAAAAGGCTTGGTGAAGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5447 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTCCTTGCCGTAGCCTC |
| Suggested primer 2: | GCTACTGCTAATGCGTGTGC |