| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.075613 |
| Chromosome: | chromosome 3 |
| Location: | 8080863 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g201200 | FAP283 | Flagellar Associated Protein 283 | 5'UTR |
| Cre03.g201250 | (1 of 1) PF01585//PF12171 - G-patch domain (G-patch) // Zinc-finger double-stranded RNA-binding (zf-C2H2_jaz) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACGCTCGGAAAACGCTCCAGGCACGAACACATCGAAAGACGATTCGG |
| Internal bar code: | AGGTTAGTCGTTGGCTCATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1275 |
| LEAP-Seq percent confirming: | 90.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCAGTTACAGGGCAAGGA |
| Suggested primer 2: | CCTCTATCTCCAGCCGCTTG |