Insertion junction: CLIP2.075623_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.075623
Chromosome:chromosome 3
Location:6751248
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g196400 EPC1 (1 of 1) K11322 - enhancer of polycomb-like protein (EPC) 5'UTR
Cre03.g196450 (1 of 1) K15178 - RNA polymerase-associated protein RTF1 (RTF1) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGCATGTCTGCCGTGTCAGTGTCTCCGTTCAGATGCTACCCACACCTG
Internal bar code:CGCTAGTTCCTTGTGGGAACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2038
LEAP-Seq percent confirming:96.875
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCTGTAGGGTTGGGAAG
Suggested primer 2:AATGTTCAGCTGTCTGCCGA