| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.075671 |
| Chromosome: | chromosome 12 |
| Location: | 9308098 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542250 | TUB1,RSP19 | (1 of 2) K07375 - tubulin beta (TUBB); beta-1 tubulin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCTCAGGGTGGCCAGTGCGGCAACCAGATTGGTGCTAAGTTCTGGGA |
| Internal bar code: | AGTTTGAAAAATCTTCAGCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 160 |
| LEAP-Seq percent confirming: | 63.0435 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCAAAACTGCTCACCCTG |
| Suggested primer 2: | CGGCACTCCTTGAATTCCCT |