Insertion junction: CLIP2.075675_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.075675
Chromosome:chromosome 2
Location:6801200
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388097 TOC80,OEP80 Putative outer membrane chloroplast protein; (1 of 2) K07277 - outer membrane protein insertion porin family (SAM50, TOB55, bamA) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTCCTCCATTAACCCACCGCCACCGCCTACCTTCCCGCACCTTCAGC
Internal bar code:CTGAGTTCGGTTGTACGCCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2727
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:106
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:106

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTCTATTGCAGCCCCAAA
Suggested primer 2:TGCACAGGTACGTCTTGGTC