| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.075675 |
| Chromosome: | chromosome 2 |
| Location: | 6801200 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388097 | TOC80,OEP80 | Putative outer membrane chloroplast protein; (1 of 2) K07277 - outer membrane protein insertion porin family (SAM50, TOB55, bamA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCCTCCTCCATTAACCCACCGCCACCGCCTACCTTCCCGCACCTTCAGC |
| Internal bar code: | CTGAGTTCGGTTGTACGCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2727 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 106 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTCTATTGCAGCCCCAAA |
| Suggested primer 2: | TGCACAGGTACGTCTTGGTC |