Insertion junction: CLIP2.075867_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.075867
Chromosome:chromosome 16
Location:3845775
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g688750 (1 of 1) K13109 - IK cytokine (IK, RED, RER) 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTATGTTTGTTCCTTTACTCTTGTTGCACCGTGGTCCATGCTGTCCATGC
Internal bar code:CTATCATTACATGCTTGTACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3925
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:32
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:32

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTGTAGGAAACGGCGGAC
Suggested primer 2:CATGTCAGCATGCACATCGG