| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.076025 |
| Chromosome: | plastome |
| Location: | 172778 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802328 | psbJ,2716960,ChreCp063 | (1 of 1) K02711 - photosystem II PsbJ protein (psbJ); photosystem II reaction center protein J | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTTATATGCTTCCCCTTCCCCTTCGGTACGTCCCCTTACGGGAATATA |
| Internal bar code: | AAAAAGTGCAGAAACCAACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 198 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCTTCAGGACGCCAGTA |
| Suggested primer 2: | ATGCCAAGCAGTTAAGGGCT |